How to Perform the Test Brief Summary * Take one of the small vials containing a pre-measured amount of activating solution and add oil sample. * Shake, and then wait a few seconds to allow liquids to separate. * Break off ends of indicator tube, and using suction bulb provided, draw clear liquid into the tube. * Lay tube on flat surface. If glycol is present, color change will occur within 15 minutes. Details of Procedure (included in kit) illustrated instructions (pdf) The indicator tubes in the kit have been pre-scratched and are ready for breaking. Locate the scratch-mark, below the plastic end and break away from mark. CAUTION: Take necessary precautions to protect fingers and eyes when breaking ends of the indicator tubes. Remove a glass vial from the carton, fill the eyedropper pipette ¾ full with the sample oil to be tested and add to the vial. For semi-quantitative results, using the concentration chart, the quantity of sample oil added should be equal to the amount of liquid in the vial. Replace the cap and shake vigorously for 20 seconds. Remove the cap, place the vial on a flat surface in an upright position, and allow the two liquids to separate. Insert one of the open ends of the indicator tube into the rubber suction bulb. Carefully squeeze the suction bulb fully between two fingers, expelling and excess liquid in the tube onto a small paper towel. (Disregard any purple color that may form on the towel.) With the suction bulb squeezed fully, insert the other open end of the tube into the bottom of the glass vial. Slowly release the fingers holding the suction bulb and allow the clear extract, from the bottom of the vial, to flow slowly to the top of the indicator tube. When the liquid just passes the top felt plug (about 1/8 inch), quickly remove the suction bulb, being careful not to allow any more liquid to enter. (Never allow any liquid to enter the suction bulb.) Remove the indicator tube from the vial, wipe the lower end with a paper towel, and place on a flat surface in a horizontal position for 15 minutes. Disregard any tan or brown color that may immediately develop, in some tests, just above, below, or in the felt plug at the lower end of the indicator tube. This band of color, 1/8 to ¾ inches long, will not increase in length, but will gradually darken to brown or black-brown and could have a dark purple base. The TEST is NEGATIVE: If no color, (other than the band described above) develops within fifteen minutes. The TEST is POSITIVE: If a faint pink color begins to develop at the base of the indicator tube. The developing pink color may begin within a few seconds, or take several minutes, it will continue to lengthen and darken to pink or shades of purple, depending on the concentration of glycol present. In higher glycol concentrations the color will develop faster, be darker, and the length of color in the tube is longer. Weaker glycol concentrations take longer to develop, are a lighter color, and the length of color in the tube will be shorter. Comparing the color developed, after 15 minutes, with the color chart, may make a Semi-Quantitative estimate of glycol concentration. A Glycol sample of 500 ppm is provided in the vial with the Red Cap. It is provided as a convenience if you wish to make a visual test of a positive sample. DO NOT ADD ANY OIL: just insert tube and proceed as described above. NOTE: BRIGHT SUN LIGHT OR HEAT MAY DAMAGE TUBES (PINK OR PURPLE COLOR DEVELOPS); HOWEVER, REGARDLESS OF STORAGE CONDITIONS, IF SILICA SAND IN TUBES IS WHITE, TUBE IS 100% OK.

Concentration Chart 1000 ppm 250 ppm 100 ppm 50 ppm